Composite

Part:BBa_K136066:Design

Designed by: Benoît D'hayer   Group: iGEM08_Paris   (2008-10-28)


araC/pBad - EnvZ*


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1532
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1532
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1532
    Illegal BglII site found at 1452
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1532
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1532
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

assembled biobricks


Source

for EnvZ* cloning from the genome of MG1655 bacteria strain mutated for EnvZ

References