Composite
Part:BBa_K136066:Design
Designed by: Benoît D'hayer Group: iGEM08_Paris (2008-10-28)
araC/pBad - EnvZ*
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1532
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1532
Illegal NheI site found at 1205 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1532
Illegal BglII site found at 1452
Illegal BamHI site found at 1144 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1532
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1532
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
assembled biobricks
Source
for EnvZ* cloning from the genome of MG1655 bacteria strain mutated for EnvZ